Saccharides, Nucleosides, Derivatives
13C, 2H, 15N, 18O Labeling

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Optical Rotation

Optical rotation measurements are made at 589 nm using a Rudolph Research Analytical Autopol® III automatic polarimeter having a 100 mm pathlength sample cell requiring about 8 mL of sample. The jacketed glass cell is routinely thermostatted at 20.0°C by a circulating water bath, but can be set to other temperatures if needed. Instrument calibration is checked periodically against a NIST-traceable quartz control plate.