Quality Assurance Assay: NMR

¹³C NMR Spectra of D-[1-¹³C]Glucose (²H₂O solvent, 75 MHz)

Spectrum A: Enriched carbons only; the labeled C1 carbons of the alpha- and beta-pyranoses are indicated.

Spectrum B: Unenriched region. The C2 signals of both pyranoses are split by the directly bonded ¹³C isotope. Two- and three-bond ¹³C-¹³C spin-couplings are observed for the C6 carbons of both pyranoses, C3 of the beta-pyranose, and C5 of the alpha-pyranose.